Harnessing alkaline-pH regulatable promoters for efficient methanol-free expression of enzymes of industrial interest in Komagataella Phaffii

Abstract Background The yeast Komagataella phaffii has become a very popular host for heterologous protein expression, very speculums-and-spreaders often based on the use of the AOX1 promoter, which becomes activated when cells are grown with methanol as a carbon source.However, the use of methanol in industrial settings is not devoid of problems,

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